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[Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing].
- Source :
-
Revista Argentina de microbiologia [Rev Argent Microbiol] 2018 Apr - Jun; Vol. 50 (2), pp. 126-130. Date of Electronic Publication: 2017 Oct 21. - Publication Year :
- 2018
-
Abstract
- Leptospirosis is a zoonosis having worldwide distribution. The objective of this work was to develop a molecular technique to differentiate pathogenic Leptospira spp. A region of adhesin ligB, present only in the pathogenic species was amplified by PCR and sequenced. ligBRpet and ligBFpet primers were used, which amplified the target DNA from pathogenic L. interrogans reference strains serovars Pomona strain Pomona, Canicola strain Hond Utrecht IV, Copenhageni strain M 20, Wolffi strain 3705, Pyrogenes strain Salinem, Hardjo strain Hardjoprajitmo, L. borgpetersenii serovar Castellonis strain Castellon 3 and 4 pathogenic strains isolated from bovines, pigs, rats and opossums. L. biflexa serovars Patoc strain Patoc I and Andamana strain Andamana were not amplified. Sequencing of the amplified products exhibited sufficient variation among serovars, which differentiates them.<br /> (Copyright © 2017 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0325-7541
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Revista Argentina de microbiologia
- Publication Type :
- Academic Journal
- Accession number :
- 29066033
- Full Text :
- https://doi.org/10.1016/j.ram.2016.11.008