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Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses.
- Source :
-
Molecular and cellular probes [Mol Cell Probes] 2018 Feb; Vol. 37, pp. 1-5. Date of Electronic Publication: 2017 Oct 18. - Publication Year :
- 2018
-
Abstract
- Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 × 10 <superscript>2</superscript> - 3.52 × 10 <superscript>3</superscript> copies/μL. We examined 60 clinical specimens and found 18 positive for respiratory viruses. Our result demonstrated that xTAG-multiplex PCR method is a high-throughput, rapid, specific and sensitive assay for use in epidemiological studies and clinical detection of avian respiratory pathogens.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
DNA Virus Infections diagnosis
DNA Virus Infections veterinary
DNA Virus Infections virology
Herpesvirus 1, Gallid genetics
Infectious bronchitis virus genetics
Influenza A virus genetics
Limit of Detection
Newcastle disease virus genetics
Poultry
Poultry Diseases virology
RNA Virus Infections diagnosis
RNA Virus Infections veterinary
RNA Virus Infections virology
Sensitivity and Specificity
Herpesvirus 1, Gallid isolation & purification
Infectious bronchitis virus isolation & purification
Influenza A virus isolation & purification
Multiplex Polymerase Chain Reaction methods
Newcastle disease virus isolation & purification
Poultry Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1194
- Volume :
- 37
- Database :
- MEDLINE
- Journal :
- Molecular and cellular probes
- Publication Type :
- Academic Journal
- Accession number :
- 29054443
- Full Text :
- https://doi.org/10.1016/j.mcp.2017.10.002