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Metabolic flux towards the (iso)flavonoid pathway in lignin modified alfalfa lines induces resistance against Fusarium oxysporum f. sp. medicaginis.
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2018 Sep; Vol. 41 (9), pp. 1997-2007. Date of Electronic Publication: 2017 Nov 21. - Publication Year :
- 2018
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Abstract
- Downregulation of lignin in alfalfa (Medicago sativa L.) is associated with increased availability of cell wall polysaccharides in plant cells. We tested transgenic alfalfa plants downregulated for Caffeoyl-CoA O-methyltransferase (CCoAOMT) against an economically important fungal disease of alfalfa, Fusarium wilt caused by Fusarium oxysporum f. sp. medicaginis, and found it more resistant to this disease. Transcriptomic and metabolomic analyses indicated that the improved disease resistance against Fusarium wilt is due to increased accumulation and/or spillover of flux towards the (iso)flavonoid pathway. Some (iso)flavonoids and their pathway intermediate compounds showed strong accumulation in CCoAOMT downregulated plants after F. oxysporum f. sp. medicaginis inoculation. The identified (iso)flavonoids, including medicarpin and 7,4'-dihydroxyflavone, inhibited the in vitro growth of F. oxysporum f. sp. medicaginis. These results suggested that the increased accumulation and/or shift/spillover of flux towards the (iso)flavonoid pathway in CCoAOMT downregulated plants is associated with induced disease resistance.<br /> (© 2017 John Wiley & Sons Ltd.)
- Subjects :
- Ascomycota pathogenicity
Disease Resistance genetics
Flavonoids genetics
Flavonoids pharmacology
Fusarium drug effects
Gene Expression Regulation, Plant
Lignin genetics
Lignin metabolism
Medicago sativa genetics
Methyltransferases genetics
Methyltransferases metabolism
Plant Diseases genetics
Plant Roots genetics
Plant Roots metabolism
Plants, Genetically Modified metabolism
Pterocarpans genetics
Pterocarpans metabolism
Pterocarpans pharmacology
Salicylic Acid metabolism
Flavonoids metabolism
Fusarium pathogenicity
Medicago sativa metabolism
Medicago sativa microbiology
Plant Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 41
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 29047109
- Full Text :
- https://doi.org/10.1111/pce.13093