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Efficient induction of apoptosis in cancer cells by paclitaxel-loaded selenium nanoparticles.
- Source :
-
Nanomedicine (London, England) [Nanomedicine (Lond)] 2017 Nov; Vol. 12 (21), pp. 2641-2651. Date of Electronic Publication: 2017 Oct 18. - Publication Year :
- 2017
-
Abstract
- Aim: To develop selenium nanoparticles (SeNPs)-based delivery systems for paclitaxel (PTX) and assess their antiproliferative efficacy against cancer cells in vitro with potential mechanistic insight.<br />Methods: Pluronic F-127 stabilized SeNPs were prepared and characterized. Effects of PTX-loaded SeNPs on lung (A549), breast (MCF7), cervical (HeLa) and colon (HT29) cancer cells were studied by viability assay complemented with flow-cytometric analyses of cell cycle, apoptosis, mitochondrial membrane potential, intracellular reactive oxygen species and caspase activity.<br />Results: PTX-loaded SeNPs demonstrated significant antiproliferative activity against cancer cells. Cell cycle analyses of PTX-SeNPs treated cells established G <subscript>2</subscript> /M phase arrest in a dose-dependent manner leading to apoptosis. Further investigation revealed disruption of mitochondrial membrane potential orchestrated with induction of reactive oxygen species leading to the activation of caspases, key players of apoptotic cell death.<br />Conclusion: Efficient induction of apoptosis in various cancer cells by PTX-loaded SeNPs, with appropriate future studies, might lead to potential anticancer strategies.
- Subjects :
- Caspases metabolism
Cell Cycle drug effects
Cell Line, Tumor
Cell Survival drug effects
Drug Liberation
Humans
Membrane Potential, Mitochondrial drug effects
Particle Size
Poloxamer chemistry
Reactive Oxygen Species metabolism
Surface Properties
Apoptosis drug effects
Drug Carriers chemistry
Nanoparticles chemistry
Paclitaxel chemistry
Paclitaxel pharmacology
Selenium Compounds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1748-6963
- Volume :
- 12
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Nanomedicine (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 29043926
- Full Text :
- https://doi.org/10.2217/nnm-2017-0189