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The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis.
- Source :
-
Scientific reports [Sci Rep] 2017 Oct 17; Vol. 7 (1), pp. 13364. Date of Electronic Publication: 2017 Oct 17. - Publication Year :
- 2017
-
Abstract
- NIPP1 is one of the major nuclear interactors of protein phosphatase PP1. The deletion of NIPP1 in mice is early embryonic lethal, which has precluded functional studies in adult tissues. Hence, we have generated an inducible NIPP1 knockout model using a tamoxifen-inducible Cre recombinase transgene. The inactivation of the NIPP1 encoding alleles (Ppp1r8) in adult mice occurred very efficiently in testis and resulted in a gradual loss of germ cells, culminating in a Sertoli-cell only phenotype. Before the overt development of this phenotype Ppp1r8 <superscript>-/-</superscript> testis showed a decreased proliferation and survival capacity of cells of the spermatogenic lineage. A reduced proliferation was also detected after the tamoxifen-induced removal of NIPP1 from cultured testis slices and isolated germ cells enriched for undifferentiated spermatogonia, hinting at a testis-intrinsic defect. Consistent with the observed phenotype, RNA sequencing identified changes in the transcript levels of cell-cycle and apoptosis regulating genes in NIPP1-depleted testis. We conclude that NIPP1 is essential for mammalian spermatogenesis because it is indispensable for the proliferation and survival of progenitor germ cells, including (un)differentiated spermatogonia.
- Subjects :
- Animals
Biomarkers
Gene Deletion
Germ Cells drug effects
Germ Cells metabolism
Immunohistochemistry
Intracellular Signaling Peptides and Proteins genetics
Male
Mammals
Mice
Mice, Knockout
Mice, Transgenic
Intracellular Signaling Peptides and Proteins metabolism
Protein Phosphatase 1 metabolism
Spermatogenesis drug effects
Spermatogenesis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29042623
- Full Text :
- https://doi.org/10.1038/s41598-017-13809-y