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Bacteria causing pyometra in bitch and queen induce neutrophil extracellular traps.
- Source :
-
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2017 Oct; Vol. 192, pp. 8-12. Date of Electronic Publication: 2017 Sep 05. - Publication Year :
- 2017
-
Abstract
- Neutrophils are capable of releasing their DNA in response to infectious agents to form neutrophil extracellular traps (NETs) to destroy pathogens. Even though pyometra in queens and bitches is a common disease, its pathogenesis is not fully understood. The aim of this study was to assess the presence of NETs in the endometrium of queens and bitches suffering from pyometra. Pyometra and normal uteri were obtained after ovariohysterectomy from adult queens and bitches in diestrus. Uterine contents were evaluated for bacterial isolation and identification and for NETs presence. Escherichia coli were isolated in 5/5 queens and 4/5 bitches, and Streptococcus spp in one bitch. Sterile glass coverslips were placed on the endometrium surface to obtain material for NETs that were evaluated by immunocytochemistry (histone, neutrophil elastase or myeloperoxidase), fluorescence microscopy or scanning electron microscopy. NETs in endometrium content were positively stained by DNA histone DAPI, myeloperoxidase and by neutrophil elastase. NETs were spread in all observed queen and bitch endometria of pyometra cases. Ultrastructure images of NETs depicted clusters of globular material with fine filaments deposited on or around thick filaments and trapped bacteria. To the best of our knowledge these are the first findings confirming NETs endometrial presence in queen and bitch pyometra. Nevertheless, the precise role of NETs in pyometra in the bitch and queen, either to contribute to the defeat of infection or to its persistence remains to be unraveled.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Dog Diseases immunology
Dogs
Endometrium microbiology
Endometrium ultrastructure
Escherichia coli pathogenicity
Female
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Pyometra microbiology
Streptococcus pathogenicity
Dog Diseases microbiology
Extracellular Traps microbiology
Pyometra veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2534
- Volume :
- 192
- Database :
- MEDLINE
- Journal :
- Veterinary immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 29042016
- Full Text :
- https://doi.org/10.1016/j.vetimm.2017.09.001