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PFKFB4 control of AKT signaling is essential for premigratory and migratory neural crest formation.
- Source :
-
Development (Cambridge, England) [Development] 2017 Nov 15; Vol. 144 (22), pp. 4183-4194. Date of Electronic Publication: 2017 Oct 16. - Publication Year :
- 2017
-
Abstract
- Neural crest (NC) specification comprises an early phase, initiating immature NC progenitors formation at neural plate stage, and a later phase at neural fold stage, resulting in a functional premigratory NC that is able to delaminate and migrate. We found that the NC gene regulatory network triggers upregulation of pfkfb4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4) during this late specification phase. As shown in previous studies, PFKFB4 controls AKT signaling in gastrulas and glycolysis rate in adult cells. Here, we focus on PFKFB4 function in NC during and after neurulation, using time-controlled or hypomorph depletions in vivo We find that PFKFB4 is essential both for specification of functional premigratory NC and for its migration. PFKFB4-depleted embryos fail to activate n-cadherin and late NC specifiers, and exhibit severe migration defects resulting in craniofacial defects. AKT signaling mediates PFKFB4 function in NC late specification, whereas both AKT signaling and glycolysis regulate migration. These findings highlight novel and essential roles of PFKFB4 activity in later stages of NC development that are wired into the NC gene regulatory network.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2017. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Epithelial-Mesenchymal Transition
Face embryology
Glycolysis
Larva
Models, Biological
Neurons cytology
Neurons metabolism
Neurulation
Skull embryology
Xenopus laevis embryology
Cell Movement
Neural Crest cytology
Phosphofructokinase-2 metabolism
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction
Xenopus Proteins metabolism
Xenopus laevis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 144
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 29038306
- Full Text :
- https://doi.org/10.1242/dev.157644