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Blockade of Interleukin 6 by Rat Anti-mouse Interleukin 6 Receptor Antibody Promotes Fracture Healing.
- Source :
-
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2017 Oct; Vol. 82 (10), pp. 1193-1199. - Publication Year :
- 2017
-
Abstract
- Level of interleukin 6 (IL-6) is associated with fracture healing. This study was performed to explore the effect of IL-6 blockade on fracture healing. Clinical serum levels of IL-6 and tumor necrosis factor-α (TNF-α) were evaluated by enzyme-linked immunosorbent assay (ELISA). For animal experiments, the IL-6 levels after fracture and treatment with rat anti-mouse IL-6 receptor antibody (MR16-1) were assessed. Then, mice were assigned into four or seven groups: control group, fracture group, isotype IgG group, and MR16-1 groups. Serum levels of IL-6 and TNF-α, relative flexural rigidity, and mRNA levels of osteoblast-specific genes were respectively assayed by ELISA, three-point bending test, and quantitative reverse transcription PCR (qRT-PCR). Serum levels of IL-6 and TNF-α after fracture in humans and mice were increased. The increase in IL-6 and TNF-α levels in murine serum was attenuated by MR16-1 treatment. The three-point bending test showed the relative flexural rigidity of the femur was decreased after fracture, whereas the decrease was alleviated by MR16-1 treatment. The qRT-PCR results demonstrated mRNA levels of osteoblast-specific genes were upregulated after fracture and then further upregulated by MR16-1 treatment in a dose-dependent manner. Collectively, the serum level of IL-6 was elevated after fracture both in clinical and murine samples. IL-6 blockade by MR16-1 promoted fracture healing, which might be associated with changes in expression of osteoblast-specific genes.
- Subjects :
- Aged
Animals
Antibodies pharmacology
Collagen Type II genetics
Collagen Type II metabolism
Collagen Type X genetics
Collagen Type X metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Femur physiology
Fracture Healing drug effects
Fractures, Bone blood
Fractures, Bone metabolism
Humans
Interleukin-6 blood
Male
Mice
Middle Aged
Rats
Receptors, Interleukin-6 immunology
Tumor Necrosis Factor-alpha blood
Up-Regulation drug effects
Antibodies immunology
Fractures, Bone pathology
Interleukin-6 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1608-3040
- Volume :
- 82
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Biochemistry. Biokhimiia
- Publication Type :
- Academic Journal
- Accession number :
- 29037140
- Full Text :
- https://doi.org/10.1134/S0006297917100121