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A disulfide bond A-like oxidoreductase is a strong candidate gene for self-incompatibility in apricot (Prunus armeniaca) pollen.
- Source :
-
Journal of experimental botany [J Exp Bot] 2017 Nov 02; Vol. 68 (18), pp. 5069-5078. - Publication Year :
- 2017
-
Abstract
- S-RNase based gametophytic self-incompatibility (SI) is a widespread prezygotic reproductive barrier in flowering plants. In the Solanaceae, Plantaginaceae and Rosaceae gametophytic SI is controlled by the pistil-specific S-RNases and the pollen S-locus F-box proteins but non-S-specific factors, namely modifiers, are also required. In apricot, Prunus armeniaca (Rosaceae), we previously mapped two pollen-part mutations that confer self-compatibility in cultivars Canino and Katy at the distal end of chromosome 3 (M-locus) unlinked to the S-locus. Here, we used high-resolution mapping to identify the M-locus with an ~134 kb segment containing ParM-1-16 genes. Gene expression analysis identified four genes preferentially expressed in anthers as modifier gene candidates, ParM-6, -7, -9 and -14. Variant calling of WGS Illumina data from Canino, Katy, and 10 self-incompatible cultivars detected a 358 bp miniature inverted-repeat transposable element (MITE) insertion in ParM-7 shared only by self-compatible apricots, supporting ParM-7 as strong candidate gene required for SI. ParM-7 encodes a disulfide bond A-like oxidoreductase protein, which we named ParMDO. The MITE insertion truncates the ParMDO ORF and produces a loss of SI function, suggesting that pollen rejection in Prunus is dependent on redox regulation. Based on phylogentic analyses we also suggest that ParMDO may have originated from a tandem duplication followed by subfunctionalization and pollen-specific expression.<br /> (© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Subjects :
- Disulfides
Genetic Loci genetics
Genotype
High-Throughput Nucleotide Sequencing
Loss of Function Mutation
Oxidoreductases genetics
Plant Proteins genetics
Plant Proteins metabolism
Pollen genetics
Pollen physiology
Prunus armeniaca genetics
Prunus armeniaca physiology
Sequence Analysis, DNA
Oxidoreductases metabolism
Pollen enzymology
Prunus armeniaca enzymology
Self-Incompatibility in Flowering Plants genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2431
- Volume :
- 68
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Journal of experimental botany
- Publication Type :
- Academic Journal
- Accession number :
- 29036710
- Full Text :
- https://doi.org/10.1093/jxb/erx336