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Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions.
- Source :
-
Scientific reports [Sci Rep] 2017 Oct 13; Vol. 7 (1), pp. 13113. Date of Electronic Publication: 2017 Oct 13. - Publication Year :
- 2017
-
Abstract
- Physiologically, lipopolysaccharide (LPS) is present in the bloodstream and can be bound to several proteins for its transport (i.e.) LPS binding protein (LBP) and plasma lipoproteins). LPS receptors CD14 and TLR-4 are constitutively expressed in the Central Nervous System (CNS). To our knowledge, LPS infiltration in CNS has not been clearly demonstrated. A naturalistic experiment with healthy rats was performed to investigate whether LPS is present with its receptors in brain. Immunofluorescences showed that lipid A and core LPS were present in circumventricular organs, choroid plexus, meningeal cells, astrocytes, tanycytes and endothelial cells. Co-localization of LPS regions with CD14/TLR-4 was found. The role of lipoprotein receptors (SR-BI, ApoER2 and LDLr) in the brain as targets for a LPS transport mechanism by plasma apolipoproteins (i.e. ApoAI) was studied. Co-localization of LPS regions with these lipoproteins markers was observed. Our results suggest that LPS infiltrates in the brain in physiological conditions, possibly, through a lipoprotein transport mechanism, and it is bound to its receptors in blood-brain interfaces.
- Subjects :
- Animals
Apolipoprotein A-I metabolism
Fluorescent Antibody Technique
Lipopolysaccharide Receptors metabolism
Male
Rats
Rats, Wistar
Scavenger Receptors, Class B metabolism
Toll-Like Receptor 4 metabolism
Antibodies immunology
Brain metabolism
Lipopolysaccharides metabolism
Lipoproteins immunology
Lipoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29030613
- Full Text :
- https://doi.org/10.1038/s41598-017-13302-6