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A novel bacteriocin from Enterococcus faecalis 478 exhibits a potent activity against vancomycin-resistant enterococci.
- Source :
-
PloS one [PLoS One] 2017 Oct 12; Vol. 12 (10), pp. e0186415. Date of Electronic Publication: 2017 Oct 12 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- The emergence of multidrug-resistant enterococci (MDRE) and particularly vancomycin-resistant enterococci (VRE) is considered a serious health problem worldwide, causing the need for new antimicrobials. The aim of this study was to discover and characterize bacteriocin against clinical isolates of MDRE and VRE. Over 10,000 bacterial isolates from water, environment and clinical samples were screened. E. faecalis strain 478 isolated from human feces produced the highest antibacterial activity against several MDRE and VRE strains. The optimum condition for bacteriocin production was cultivation in MRS broth at 37°C, pH 5-6 for 16 hours. The bacteriocin-like substance produced from E. faecalis strain EF478 was stable at 60°C for at least 1 hour and retained its antimicrobial activity after storage at -20°C for 1 year, at 4°C for 6 months, and at 25°C for 2 months. A nano-HPLC electrospray ionization multi-stage tandem mass spectrometry (nLC-ESI-MS/MS) analysis showed that the amino acid sequences of the bacteriocin-like substance was similar to serine protease of E. faecalis, gi|488296663 (NCBI database), which has never been reported as a bacteriocin. This study reported a novel bacteriocin with high antibacterial activity against VRE and MDRE.
- Subjects :
- Amino Acid Sequence
Anti-Infective Agents chemistry
Anti-Infective Agents isolation & purification
Bacteriocins isolation & purification
Bacteriocins pharmacology
DNA chemistry
DNA isolation & purification
DNA metabolism
Enterococcus faecalis isolation & purification
Feces microbiology
Humans
Hydrogen-Ion Concentration
Molecular Sequence Data
Nanotechnology
Protein Structure, Tertiary
Sequence Alignment
Sequence Analysis, DNA
Serine Proteases chemistry
Spectrometry, Mass, Electrospray Ionization
Temperature
Anti-Infective Agents pharmacology
Bacteriocins chemistry
Enterococcus faecalis metabolism
Vancomycin-Resistant Enterococci drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 29023515
- Full Text :
- https://doi.org/10.1371/journal.pone.0186415