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Detection and molecular characterization of equine infectious anemia virus in Mongolian horses.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2017 Nov 17; Vol. 79 (11), pp. 1884-1888. Date of Electronic Publication: 2017 Oct 11. - Publication Year :
- 2017
-
Abstract
- The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94.0% identity with the third. These two independent Mongolian EIAV sequences were retained functional motifs, with no dramatic changes but some variability in the U5 region; they were clustered with genotypes from European countries but not with those from China, U.S.A., or Japan.
- Subjects :
- Animals
DNA, Viral
Equine Infectious Anemia epidemiology
Genetic Variation
Genome, Viral
Horses
Mongolia epidemiology
Polymerase Chain Reaction veterinary
Sequence Analysis, DNA
Seroepidemiologic Studies
Equine Infectious Anemia virology
Infectious Anemia Virus, Equine genetics
Infectious Anemia Virus, Equine isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 79
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 29021424
- Full Text :
- https://doi.org/10.1292/jvms.17-0202