Identification of CfiA coding genes in Bacteroides fragilis isolates recovered in Argentina. Inconsistencies in CfiA organization and nomenclature.

Authors :: Litterio MR
Cejas D
Gutkind G
Radice M
Source :: Anaerobe [Anaerobe] 2017 Dec; Vol. 48, pp. 257-261. Date of Electronic Publication: 2017 Oct 07.
Publication Year :: 2017
Abstract: CfiA (CcrA) metallo-β-lactamase is the main carbapenem resistance mechanism in B. fragilis. From cfiA positive isolates detected in a previous surveillance study, 3 displayed resistance to imipenem while the remaining were susceptible. The aim of this study was to identify the cfiA alleles and to analyze the presence of IS elements in their upstream regions. CfiA-1, CfiA-4, CfiA-13, CfiA-19 and CfiA-22 were detected. IS elements belonging to IS21 family and IS942 group were identified upstream to cfiA in the 3 imipenem resistant isolates. We present an exhaustive analysis of cfiA/CfiA registers in databases, illustrating the inconsistencies in both organization and nomenclature. According to this analysis CfiA family comprises nowadays 15 different CfiA variants coded by 24 cfiA sequences. Curation of CfiA database is mandatory, if not new cfiA admission at GenBank will contribute to make this classification more complex.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Subjects :: Alleles
Anti-Bacterial Agents pharmacology
Argentina epidemiology
Bacteroides Infections epidemiology
Bacteroides fragilis drug effects
Bacteroides fragilis isolation & purification
Humans
Microbial Sensitivity Tests
Phylogeny
Public Health Surveillance
Bacterial Proteins genetics
Bacteroides Infections microbiology
Bacteroides fragilis classification
Bacteroides fragilis genetics
Open Reading Frames
beta-Lactamases genetics

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