Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China.

A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1 ^T , was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m ^-2 ). Based on 16S rRNA gene sequence comparisons, strain 6.1 ^T shows high similarity to Deinococcus citri DSM 24791 ^T (98.1%) and Deinococcus gobiensis I-0 ^T (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C _16:1 ω7c and/or iso-C _15:0 2-OH), followed by iso-C _17:1 ω9c and C _16:0 . The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA-DNA hybridization between strain 6.1 ^T and D. citri DSM 24791 ^T was 45.6 ± 7.1% and with D. gobiensis I-O ^T was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1 ^T represents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1 ^T (= CGMCC 1.15053 ^T  = KCTC 33744 ^T ).