Back to Search
Start Over
Assessment of tobacco heating product THP1.0. Part 5: In vitro dosimetric and cytotoxic assessment.
- Source :
-
Regulatory toxicology and pharmacology : RTP [Regul Toxicol Pharmacol] 2018 Mar; Vol. 93, pp. 52-61. Date of Electronic Publication: 2017 Oct 01. - Publication Year :
- 2018
-
Abstract
- Tobacco heating products (THPs) represent a subset of the next-generation nicotine and tobacco product category, in which tobacco is typically heated at temperatures of 250-350 °C, thereby avoiding many of the harmful combustion-related toxicant emissions of conventional cigarettes. In this study, we have assessed aerosol generation and cytotoxicity from two commercially available THPs, THP1.0 and THS, relative to tobacco smoke from 3R4F reference cigarettes, using an adapted Borgwaldt RM20S Smoking Machine. Quantification of nicotine in the exposed cell-culture media showed greater delivery of nicotine from both THPs than from the cigarette. Using Neutral Red Uptake assay, THPs demonstrated reduced in vitro cytotoxicity in H292 human bronchial epithelial cells as compared with 3R4F cigarette exposure at the air-liquid interface (p < 0.0001). Both THPs demonstrated a statistically similar reduction in biological response, with >87% viability relative to 3R4F at a common aerosol dilution (1:40, aerosol:air). A similar response was observed when plotted against nicotine; a statistical difference between 3R4F and THPs (p < 0.0001) and no difference between the THPs (p = 0.0186). This pre-clinical in vitro biological testing forms part of a larger package of data to help assess the safety and risk reduction potential of next-generation tobacco products relative to cigarettes, using a weight of evidence approach.<br /> (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Cell Line
Cell Survival drug effects
Cell Survival physiology
Cytotoxins pharmacology
Dose-Response Relationship, Drug
Humans
Nicotine pharmacology
Respiratory Mucosa drug effects
Respiratory Mucosa physiology
Cytotoxins analysis
Electronic Nicotine Delivery Systems methods
Heating methods
Nicotine analysis
Tobacco Products analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0295
- Volume :
- 93
- Database :
- MEDLINE
- Journal :
- Regulatory toxicology and pharmacology : RTP
- Publication Type :
- Academic Journal
- Accession number :
- 28987911
- Full Text :
- https://doi.org/10.1016/j.yrtph.2017.09.016