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Phenylobacterium deserti sp. nov., isolated from desert soil.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2017 Nov; Vol. 67 (11), pp. 4722-4727. Date of Electronic Publication: 2017 Oct 06. - Publication Year :
- 2017
-
Abstract
- A novel bacterial strain, designated YIM 73061 <superscript>T</superscript> , was isolated from the Cholistan desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Phenylobacterium conjunctum FWC21 <superscript>T</superscript> (97.6 %), Phenylobacterium lituiforme FaiI3 <superscript>T</superscript> (97.4 %), Phenylobacteriumcomposti 4T-6 <superscript>T</superscript> (97.0 %) and Phenylobacterium aquaticum W2-3-4 <superscript>T</superscript> (96.8 %). Cells were Gram-stain-negative, aerobic and motile rods that formed orange colonies. The strain was oxidase- and catalase-positive. Growth occurred at 20-40 °C (optimum, 30-37 °C) at pH 5.0-8.0 (optimum, pH 7.0) and with 0-1 % (w/v) NaCl (optimum, 0-0.5 %). The major cellular fatty acids (>10 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipid profile consisted of phosphatidylglycerol and four unidentified glycolipids. The major isoprenoid quinone was ubiquinone-10 (Q-10). The G+C content of the genomic DNA was 66.8 mol%. Strain YIM 73061 <superscript>T</superscript> showed low levels of DNA-DNA relatedness to P. conjunctum FWC21 <superscript>T</superscript> (27.2±2.6 %), P. lituiforme FaiI3 <superscript>T</superscript> (24.6±1.1 %) and P.composti 4T-6 <superscript>T</superscript> (18.4±3.1 %). On the basis of phylogenetic inference, chemotaxonomic characteristics and phenotypic data, strain YIM 73061 <superscript>T</superscript> should be classified as representing a novel species, for which the name Phenylobacterium deserti sp. nov. is proposed. The type strain is YIM 73061 <superscript>T</superscript> (=DSM 103871 <superscript>T</superscript> =CCTCC AB 2016297 <superscript>T</superscript> ).
- Subjects :
- Bacterial Typing Techniques
Base Composition
Caulobacteraceae genetics
Caulobacteraceae isolation & purification
DNA, Bacterial genetics
Fatty Acids chemistry
Glycolipids chemistry
Nucleic Acid Hybridization
Pakistan
Phospholipids chemistry
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Ubiquinone chemistry
Caulobacteraceae classification
Desert Climate
Phylogeny
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 67
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28984224
- Full Text :
- https://doi.org/10.1099/ijsem.0.002366