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Mechanistic understanding of Phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2017 Nov; Vol. 101 (22), pp. 8223-8236. Date of Electronic Publication: 2017 Oct 05. - Publication Year :
- 2017
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Abstract
- Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors' production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P. aeruginosa PAO1. Our study revealed that PLA inhibited the expression of virulence factors such as pyocyanin, protease, and rhamnolipids that are involved in the biofilm formation of P. aeruginosa PAO1. Swarming motility, another important criterion for biofilm formation of P. aeruginosa PAO1, was also inhibited by PLA. Gene expression, mass spectrometric, functional complementation assays, and in silico data indicated that the quorum quenching and biofilm inhibitory activities of PLA are attributed to its ability to interact with P. aeruginosa QS receptors. PLA antagonistically binds to QS receptors RhlR and PqsR with a higher affinity than its cognate ligands N-butyryl-L-homoserine lactone (C <subscript>4</subscript> -HSL) and 2-heptyl-3,4-dihydroxyquinoline (PQS; Pseudomonas quinolone signal). Using an in vivo intraperitoneal catheter-associated medaka fish infection model, we proved that PLA inhibited the initial attachment of P. aeruginosa PAO1 on implanted catheter tubes. Our in vitro and in vivo results revealed the potential of PLA as anti-biofilm compound against P. aeruginosa.
- Subjects :
- 4-Butyrolactone analogs & derivatives
4-Butyrolactone metabolism
Animals
Catheters microbiology
Computer Simulation
Disease Models, Animal
Gene Expression
Genetic Complementation Test
Lactobacillus metabolism
Oryzias microbiology
Pseudomonas Infections drug therapy
Pseudomonas Infections microbiology
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa pathogenicity
Pyocyanine metabolism
Virulence Factors
Anti-Bacterial Agents pharmacology
Biofilms drug effects
Lactates pharmacology
Pseudomonas aeruginosa drug effects
Quorum Sensing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 101
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 28983655
- Full Text :
- https://doi.org/10.1007/s00253-017-8546-4