Liquid biopsy in the diagnosis of HPV DNA in breast lesions.

Authors :: Carolis S
Pellegrini A
Santini D
Ceccarelli C
De Leo A
Alessandrini F
Arienti C
Pignatta S
Tesei A
Mantovani V
Zamagni C
Taffurelli M
Sansone P
Bonafé M
Cricca M
Source :: Future microbiology [Future Microbiol] 2018 Feb; Vol. 13, pp. 187-194. Date of Electronic Publication: 2017 Oct 04.
Publication Year :: 2018
Abstract: Aim: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens.<br />Materials & Methods: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types.<br />Results: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles.<br />Conclusion: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.