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The neurotrophic effects of different human dental mesenchymal stem cells.
- Source :
-
Scientific reports [Sci Rep] 2017 Oct 03; Vol. 7 (1), pp. 12605. Date of Electronic Publication: 2017 Oct 03. - Publication Year :
- 2017
-
Abstract
- The current gold standard treatment for peripheral nerve injury is nerve grafting but this has disadvantages such as donor site morbidity. New techniques focus on replacing these grafts with nerve conduits enhanced with growth factors and/or various cell types such as mesenchymal stem cells (MSCs). Dental-MSCs (D-MSCs) including stem cells obtained from apical papilla (SCAP), dental pulp stem cells (DPSC), and periodontal ligament stem cells (PDLSC) are potential sources of MSCs for nerve repair. Here we present the characterization of various D-MSCs from the same human donors for peripheral nerve regeneration. SCAP, DPSC and PDLSC expressed BDNF, GDNF, NGF, NTF3, ANGPT1 and VEGFA growth factor transcripts. Conditioned media from D-MSCs enhanced neurite outgrowth in an in vitro assay. Application of neutralizing antibodies showed that brain derived neurotrophic factor plays an important mechanistic role by which the D-MSCs stimulate neurite outgrowth. SCAP, DPSC and PDLSC were used to treat a 10 mm nerve gap defect in a rat sciatic nerve injury model. All the stem cell types significantly enhanced axon regeneration after two weeks and showed neuroprotective effects on the dorsal root ganglia neurons. Overall the results suggested SCAP to be the optimal dental stem cell type for peripheral nerve repair.
- Subjects :
- Animals
Antibodies, Neutralizing pharmacology
Axons drug effects
Brain-Derived Neurotrophic Factor genetics
Culture Media, Conditioned pharmacology
Dental Papilla cytology
Dental Pulp cytology
Humans
Mesenchymal Stem Cells drug effects
Nerve Regeneration drug effects
Nerve Tissue cytology
Nerve Tissue growth & development
Neuronal Outgrowth drug effects
Periodontal Ligament cytology
Peripheral Nerve Injuries pathology
Rats
Sciatic Neuropathy pathology
Tissue Transplantation methods
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells cytology
Peripheral Nerve Injuries therapy
Sciatic Neuropathy therapy
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28974767
- Full Text :
- https://doi.org/10.1038/s41598-017-12969-1