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Replication of Zika Virus in Human Prostate Cells: A Potential Source of Sexually Transmitted Virus.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2018 Jan 30; Vol. 217 (4), pp. 538-547. - Publication Year :
- 2018
-
Abstract
- Background: While Zika virus (ZIKV) is mainly transmitted by mosquitoes, numerous cases of sexual transmission have been reported during recent outbreaks. Little is known about which host cell types or entry factors aid in mediating this sexual transmission.<br />Methods: In this study, we investigated ZIKV cell tropism by infecting 2 types of human prostate cells with 3 contemporary ZIKV isolates from persons infected in the Americas. We used real-time quantitative polymerase chain reaction and immunofluorescence analyses to measure infection and flow cytometry to detect entry factor expression.<br />Results: Here we show that ZIKV infects, replicates, and produces infectious virus in prostate stromal mesenchymal stem cells, epithelial cells, and organoids made with a combination of these cells. We also show that prostate cells express several well-characterized flavivirus attachment factors. In contrast, dengue virus does not infect or does not replicate in these prostate cells, although it is known to use similar receptors.<br />Conclusions: Our results indicate that ZIKV favors infection of stromal cells more so than epithelial cells in organoids, possibly indicating a preference for stem cells in general. Overall, these results suggest that ZIKV replication occurs in the human prostate and can account for ZIKV secretion in semen, thus leading to sexual transmission.<br /> (© The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
- Subjects :
- Americas
Flow Cytometry
Humans
Male
Microscopy, Fluorescence
Real-Time Polymerase Chain Reaction
Virus Cultivation
Zika Virus isolation & purification
Zika Virus Infection virology
Epithelial Cells virology
Mesenchymal Stem Cells virology
Prostate virology
Viral Tropism
Virus Replication
Zika Virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 217
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 28968863
- Full Text :
- https://doi.org/10.1093/infdis/jix436