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Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells.

Authors :
Duc HN
Ren X
Source :
Bio-protocol [Bio Protoc] 2017 Sep 05; Vol. 7 (17).
Publication Year :
2017

Abstract

HaloTag has been widely used to label proteins in vitro and in vivo (Los et al ., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al ., 2016).

Details

Language :
English
ISSN :
2331-8325
Volume :
7
Issue :
17
Database :
MEDLINE
Journal :
Bio-protocol
Publication Type :
Academic Journal
Accession number :
28966948
Full Text :
https://doi.org/10.21769/BioProtoc.2526