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Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2017 Nov 17; Vol. 292 (46), pp. 19122-19132. Date of Electronic Publication: 2017 Sep 21. - Publication Year :
- 2017
-
Abstract
- We report here an approach to redirecting somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts to epithelial-like cells with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting epithelial-like cells that can be further reprogrammed to endodermal progenitor cells. Interestingly, these cells can self-renew in vitro and further differentiate into albumin-producing hepatocytes that can rescue mice from acute liver injury. Our results demonstrate a rational approach to convert mouse embryonic fibroblasts to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells.<br /> (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 292
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28935668
- Full Text :
- https://doi.org/10.1074/jbc.M117.812537