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Label-Free and High-Throughput Detection of Biomolecular Interactions Using a Flatbed Scanner Biosensor.

Authors :
Aygun U
Avci O
Seymour E
Urey H
Ünlü MS
Ozkumur AY
Source :
ACS sensors [ACS Sens] 2017 Oct 27; Vol. 2 (10), pp. 1424-1429. Date of Electronic Publication: 2017 Oct 04.
Publication Year :
2017

Abstract

Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (∼100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications. Using this sensor, we report detection of DNA hybridization and DNA directed antibody immobilization on label-free microarrays with a noise floor of ∼30 pg/mm <superscript>2</superscript> , and a scan speed of 5 s (50 s for 10 frames averaged) for a 2 mm × 2 mm area. This novel system may be used as a standalone label-free sensor especially in low-resource settings, as well as for quality control and calibration of microarrays in existing fluorescence-based DNA and protein detection platforms.

Details

Language :
English
ISSN :
2379-3694
Volume :
2
Issue :
10
Database :
MEDLINE
Journal :
ACS sensors
Publication Type :
Academic Journal
Accession number :
28929734
Full Text :
https://doi.org/10.1021/acssensors.7b00263