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Label-Free and High-Throughput Detection of Biomolecular Interactions Using a Flatbed Scanner Biosensor.
- Source :
-
ACS sensors [ACS Sens] 2017 Oct 27; Vol. 2 (10), pp. 1424-1429. Date of Electronic Publication: 2017 Oct 04. - Publication Year :
- 2017
-
Abstract
- Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (∼100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications. Using this sensor, we report detection of DNA hybridization and DNA directed antibody immobilization on label-free microarrays with a noise floor of ∼30 pg/mm <superscript>2</superscript> , and a scan speed of 5 s (50 s for 10 frames averaged) for a 2 mm × 2 mm area. This novel system may be used as a standalone label-free sensor especially in low-resource settings, as well as for quality control and calibration of microarrays in existing fluorescence-based DNA and protein detection platforms.
- Subjects :
- Biosensing Techniques methods
DNA chemistry
DNA metabolism
Fluorescence
High-Throughput Screening Assays methods
Humans
Interferometry
Light
Microarray Analysis methods
Nucleic Acid Hybridization
Biosensing Techniques instrumentation
DNA analysis
High-Throughput Screening Assays instrumentation
Microarray Analysis instrumentation
Optical Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 2379-3694
- Volume :
- 2
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- ACS sensors
- Publication Type :
- Academic Journal
- Accession number :
- 28929734
- Full Text :
- https://doi.org/10.1021/acssensors.7b00263