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Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis.
- Source :
-
Journal of gastroenterology [J Gastroenterol] 2018 Mar; Vol. 53 (3), pp. 449-460. Date of Electronic Publication: 2017 Sep 18. - Publication Year :
- 2018
-
Abstract
- Background: Pathophysiology of type 1 autoimmune pancreatitis (AIP) is still unclear. We previously reported that M2 macrophages might play an important role in type 1 AIP. Recently, it has been reported that basophils regulate differentiation to M2 macrophages. In this study, we investigated basophils from the pancreatic tissue and peripheral blood of individuals with type 1 AIP.<br />Methods: By using immunohistochemistry, we investigated basophils in pancreatic tissue from 13 patients with type 1 AIP and examined expression of toll-like receptors (TLRs) by these cells. Additionally, we obtained peripheral blood samples from 27 healthy subjects, 40 patients with type 1 AIP, 8 patients with alcoholic chronic pancreatitis, 10 patients with bronchial asthma, and 10 patients with atopic dermatitis, and analyzed activation of basophils by stimulating them with ligands of TLR1-9. We also compared TLR expression in basophils from the tissue and blood samples.<br />Results: Basophils were detected in pancreatic tissues from 10 of 13 patients with type 1 AIP. Flow cytometric analysis revealed that the ratios of basophils activated by TLR4 stimulation in type 1 AIP (9.875 ± 1.148%) and atopic dermatitis (11.768 ± 1.899%) were significantly higher than those in healthy subjects (5.051 ± 0.730%; P < 0.05). Levels of basophils activated by TLR2 stimulation were higher in seven type 1 AIP cases. Furthermore, stimulation of TLR2 and/or TLR4, which were expressed by basophils in pancreas, activated basophils in peripheral blood.<br />Conclusions: Basophils activated via TLR signaling may play an important role in the pathophysiology of type 1 AIP.
- Subjects :
- Adult
Aged
Aged, 80 and over
Autoimmune Diseases physiopathology
Female
Hospitals, University
Humans
Immunoglobulin G blood
Japan
Male
Middle Aged
Pancreatitis physiopathology
Signal Transduction
Autoimmune Diseases metabolism
Basophils metabolism
Pancreatitis immunology
Pancreatitis metabolism
Toll-Like Receptor 2 metabolism
Toll-Like Receptor 4 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1435-5922
- Volume :
- 53
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 28921377
- Full Text :
- https://doi.org/10.1007/s00535-017-1390-6