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Allobranchiibius huperziae gen. nov., sp. nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.).
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2017 Oct; Vol. 67 (10), pp. 4210-4215. Date of Electronic Publication: 2017 Sep 18. - Publication Year :
- 2017
-
Abstract
- A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077 <superscript>T</superscript> , was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.) collected from Sichuan Province, south-west China. The peptidoglycan type of strain CPCC 204077 <superscript>T</superscript> was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge. Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates. MK-8(H4) was the only menaquinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid. The major fatty acid was iso-C16 : 0. The genomic DNA G+C content was 71.0 mol%. The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077 <superscript>T</superscript> stood for a distinct lineage within the family Dermacoccaceaealongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717 <superscript>T</superscript> (95.0 %), Calidifontibacter indicus PC IW02 <superscript>T</superscript> (95.0 %), Barrientosiimonas humi 39 <superscript>T</superscript> (94.9 %) and Demetria terragena HKI 0089 <superscript>T</superscript> (94.7 %), and less than 94.7 % sequence similarities to all other species. Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceaefamily-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern. Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077 <superscript>T</superscript> represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp. nov. Strain CPCC 204077 <superscript>T</superscript> (=NBRC 110719 <superscript>T</superscript> =DSM 29531 <superscript>T</superscript> ) is the type strain of the type species.
- Subjects :
- Actinomycetales genetics
Actinomycetales isolation & purification
Bacterial Typing Techniques
Base Composition
Cell Wall chemistry
China
DNA, Bacterial genetics
Fatty Acids chemistry
Glycolipids chemistry
Peptidoglycan chemistry
Phospholipids chemistry
Plants, Medicinal microbiology
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Actinomycetales classification
Huperzia microbiology
Phylogeny
Plant Roots microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 67
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28920829
- Full Text :
- https://doi.org/10.1099/ijsem.0.002284