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Migratory capacity and function of dendritic cells from mesenteric afferent lymph nodes after feeding a single dose of vitamin A.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2017 Nov; Vol. 49, pp. 110-116. Date of Electronic Publication: 2017 Aug 24. - Publication Year :
- 2017
-
Abstract
- Lamina propria dendritic cells (DCs) have a permanent turnover with constitutive migration to mesenteric lymph nodes and replenishment by progenitors. Luminal bacteria and dietary constituents provide key signals that endow DCs their unique properties in vivo. Taking into account that the intestinal immune system is greatly influenced by retinoids, we evaluated in B6 mice 3, 8, 16 and 24 h after feeding a single dose of vitamin A phenotype and function of cells present in mesenteric afferent lymph nodes as well as signals involved in migration. We studied the frequency of CD11c+MHC-II+CD103+CD86+ and RALDH+ DCs by flow cytometry, we determined CCL-21 and D6 levels in tissue homogenates by Western blot, and we co-cultured cells isolated from afferent lymphatics with sorted CD4+ lymphocytes to assess Foxp-3 induction and homing receptor expression. Sixteen hours after vitamin A administration, DCs isolated from afferent lymphatics were able to induce homing receptors and Foxp3 expression in CD4+ lymphocytes. Our results show that a single dose of vitamin A generated a stream of signals and amplified the tolerogenic activity of DCs migrating to lymphoid tissue.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antigens, CD metabolism
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes immunology
Cell Movement
Cells, Cultured
Coculture Techniques
Dendritic Cells cytology
Dendritic Cells immunology
Female
Forkhead Transcription Factors genetics
Forkhead Transcription Factors metabolism
Immune Tolerance
Lymph cytology
Lymph immunology
Lymph metabolism
Lymph Nodes cytology
Lymph Nodes immunology
Lymph Nodes metabolism
Mesentery
Mice, Inbred C57BL
Mice, Transgenic
Receptors, Lymphocyte Homing genetics
Receptors, Lymphocyte Homing metabolism
Specific Pathogen-Free Organisms
CD4-Positive T-Lymphocytes metabolism
Dendritic Cells metabolism
Dietary Supplements
Forkhead Transcription Factors agonists
Gene Expression Regulation
Receptors, Lymphocyte Homing agonists
Vitamin A administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4847
- Volume :
- 49
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28917953
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2017.08.005