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Increased expression of NLRP3 inflammasome in placentas from pregnant women with severe preeclampsia.
- Source :
-
Journal of reproductive immunology [J Reprod Immunol] 2017 Sep; Vol. 123, pp. 40-47. Date of Electronic Publication: 2017 Sep 06. - Publication Year :
- 2017
-
Abstract
- Preeclampsia is a pregnancy disorder characterized by imbalance between pro- and anti-inflammatory cytokines associated with high plasma levels of uric acid and Interleukin-1 beta (IL-1β). The inflammasome is a protein complex that mediates innate immune responses via caspase-1 activation promoting secretion of IL-1β and IL-18 in their active forms, and also release of the high-mobility group box 1 protein (HMGB1). As the placenta seems to play an important role in the pathogenesis of PE, the present study investigated the expression of genes and proteins related to the inflammasome in placentas from pregnant women with severe preeclampsia. Placental tissue was collected from 20 normotensive pregnant women and 20 preeclamptic women, and inflammasome components, NLRP3 (NOD-like receptor family, pyrin domain-containing protein 3), caspase-1, IL-1β and IL-18, as well as tumor necrosis factor-alpha (TNF-α) and HMGB1 were evaluated by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and also quantified by reverse transcription-qPCR (RT-qPCR). Compared with normotensive pregnant women, placenta from women with PE showed a significant increase in NLRP3, caspase-1, IL-1β, TNF-α and HMGB1 mRNA. Immunohistochemical staining of NLRP3, caspase-1, IL-1β and TNF-α in placental villi, as well as the levels of caspase-1, IL-1β, TNF-α and HMGB1 in placental homogenate were significantly higher in the preeclamptic group than in the normotensive group. However, mRNA expression of IL-18 and its protein concentrations were lower in placentas from preeclamptic women. The results suggest that placentas from pregnant women with preeclampsia show higher expression of NLRP3 inflammasome, which may be involved in the exaggerated inflammatory state in preeclampsia.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Adolescent
Adult
Cytokines metabolism
Disease Progression
Female
Gene Expression Regulation
HMGB1 Protein metabolism
Humans
Immunity, Innate
Inflammasomes metabolism
Inflammation Mediators metabolism
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
Pre-Eclampsia immunology
Pregnancy
Prospective Studies
Young Adult
Inflammasomes genetics
NLR Family, Pyrin Domain-Containing 3 Protein genetics
Placenta physiology
Pre-Eclampsia genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7603
- Volume :
- 123
- Database :
- MEDLINE
- Journal :
- Journal of reproductive immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28915449
- Full Text :
- https://doi.org/10.1016/j.jri.2017.09.002