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Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PM 2.5 , Budesonide, and Cotreated Exposures.
- Source :
-
Mediators of inflammation [Mediators Inflamm] 2017; Vol. 2017, pp. 6827194. Date of Electronic Publication: 2017 Aug 16. - Publication Year :
- 2017
-
Abstract
- ATP-binding cassette subfamily C (ABCC) genes code for phase III metabolism proteins that translocate xenobiotic (e.g., particulate matter 2.5 (PM <subscript>2.5</subscript> )) and drug metabolites outside the cells. IL-6 secretion is related with the activation of the ABCC transporters. This study assesses ABCC1-4 gene expression changes and proinflammatory cytokine (IL-6, IL-8) release in human bronchial epithelial cells (BEAS-2B) exposed to PM <subscript>2.5</subscript> organic extract, budesonide (BUD, used to control inflammation in asthmatic patients), and a cotreatment (Co-T: PM <subscript>2.5</subscript> and BUD). A real-time PCR assay shows that ABCC1 was upregulated in BEAS-2B exposed after 6 and 7 hr to PM <subscript>2.5</subscript> extract or BUD but downregulated after 6 hr of the Co-T. ABCC3 was downregulated after 6 hr of BUD and upregulated after 6 hr of the Co-T exposures. ABCC4 was upregulated after 5 hr of PM <subscript>2.5</subscript> extract, BUD, and the Co-T exposures. The cytokine assay revealed an increase in IL-6 release by BEAS-2B exposed after 5 hr to PM <subscript>2.5</subscript> extract, BUD, and the Co-T. At 7 hr, the Co-T decreases IL-6 release and IL-8 at 6 hr. In conclusion, the cotreatment showed an opposite effect on exposed BEAS-2B as compared with BUD. The results suggest an interference of the BUD therapeutic potential by PM <subscript>2.5</subscript> .
- Subjects :
- Bronchi cytology
Cell Line
Cell Survival drug effects
Epithelial Cells drug effects
Humans
Interleukin-6 metabolism
Interleukin-8 metabolism
Multidrug Resistance-Associated Protein 2
Multidrug Resistance-Associated Proteins metabolism
Polymerase Chain Reaction
Budesonide toxicity
Particulate Matter toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1861
- Volume :
- 2017
- Database :
- MEDLINE
- Journal :
- Mediators of inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 28900313
- Full Text :
- https://doi.org/10.1155/2017/6827194