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[Optimal Artificial Mini-Introns for Transgenic Expression in the Cells of Mice and Hamsters].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2017 Jul-Aug; Vol. 51 (4), pp. 671-676. - Publication Year :
- 2017
-
Abstract
- Introns can frequently enhance transgene expression, and sometimes they are absolutely substantial. Based on an analysis of murine genes, in which mRNA does not have alternative splicing, a universal design of the efficiently spliced artificial introns of small sizes has been proposed. These introns are shown to be efficiently spliced in CHO cells from hamster ovaries. The proposed strategy can be used to include introns in cDNA, which would elevate the production of recombinant proteins in cell culture, as well as in transgenic animals.
- Subjects :
- Animals
Animals, Genetically Modified
Base Sequence
CHO Cells
Cricetulus
DNA, Complementary genetics
DNA, Complementary metabolism
Exons
Mice
Nucleotides genetics
Nucleotides metabolism
RNA, Messenger metabolism
Transcription, Genetic
Alternative Splicing
Genetic Engineering methods
Introns
RNA, Messenger genetics
Transgenes
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 28900086
- Full Text :
- https://doi.org/10.7868/S0026898417040176