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[CRISPR/CAS9, the King of Genome Editing Tools].

Authors :
Bannikov AV
Lavrov AV
Source :
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2017 Jul-Aug; Vol. 51 (4), pp. 582-594.
Publication Year :
2017

Abstract

The discovery of CRISPR/Cas9 brought a hope for having an efficient, reliable, and readily available tool for genome editing. CRISPR/Cas9 is certainly easy to use, while its efficiency and reliability remain the focus of studies. The review describes the general principles of the organization and function of Cas nucleases and a number of important issues to be considered while planning genome editing experiments with CRISPR/Cas9. The issues include evaluation of the efficiency and specificity for Cas9, sgRNA selection, Cas9 variants designed artificially, and use of homologous recombination and nonhomologous end joining in DNA editing.

Details

Language :
Russian
ISSN :
0026-8984
Volume :
51
Issue :
4
Database :
MEDLINE
Journal :
Molekuliarnaia biologiia
Publication Type :
Academic Journal
Accession number :
28900076
Full Text :
https://doi.org/10.7868/S0026898417040036