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Formulation of an optimized synergistic enzyme cocktail, HoloMix, for effective degradation of various pre-treated hardwoods.
- Source :
-
Bioresource technology [Bioresour Technol] 2017 Dec; Vol. 245 (Pt A), pp. 52-65. Date of Electronic Publication: 2017 Sep 01. - Publication Year :
- 2017
-
Abstract
- In this study, two selected hardwoods were subjected to sodium chlorite delignification and steam explosion, and the impact of pre-treatments on synergistic enzymatic saccharification evaluated. A cellulolytic core-set, CelMix, and a xylanolytic core-set, XynMix, optimised for glucose and xylose release, respectively, were used to formulate HoloMix cocktail for optimal saccharification of various pre-treated hardwoods. For delignified biomass, the optimized HoloMix consisted of 75%:25% protein dosage, CelMix: XynMix, while for untreated and steam exploded biomass the HoloMix consisted of 93.75%:6.25% protein dosage. Saccharification by HoloMix (27.5mgprotein/gbiomass) for 24h achieved 70-100% sugar yields. Pre-treatment of the hardwoods (especially those with a higher proportion of lignin) with a laccase, improved saccharification by HoloMix. This study provided insights into enzymatic hydrolysis of various pre-treated hardwood substrates and showed the same lignocellulolytic cocktail comparable to/if not better than commercial enzyme preparations can be used to efficiently hydrolyse different hardwood species.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Hydrolysis
Lignin
Steam
Biomass
Cellulase
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 245
- Issue :
- Pt A
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 28892706
- Full Text :
- https://doi.org/10.1016/j.biortech.2017.08.186