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Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability.
- Source :
-
Environmental microbiology [Environ Microbiol] 2017 Oct; Vol. 19 (10), pp. 4326-4348. Date of Electronic Publication: 2017 Oct 02. - Publication Year :
- 2017
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Abstract
- Campylobacter jejuni, the most frequent cause of food-borne bacterial gastroenteritis worldwide, is a microaerophile that has to survive high environmental oxygen tensions, adapt to oxygen limitation in the intestine and resist host oxidative attack. Here, oxygen-dependent changes in C. jejuni physiology were studied at constant growth rate using carbon (serine)-limited continuous chemostat cultures. We show that a perceived aerobiosis scale can be calibrated by the acetate excretion flux, which becomes zero when metabolism is fully aerobic (100% aerobiosis). Transcriptome changes in a downshift experiment from 150% to 40% aerobiosis revealed many novel oxygen-regulated genes and highlighted re-modelling of the electron transport chains. A label-free proteomic analysis showed that at 40% aerobiosis, many proteins involved in host colonisation (e.g., PorA, CadF, FlpA, CjkT) became more abundant. PorA abundance increased steeply below 100% aerobiosis. In contrast, several citric-acid cycle enzymes, the peptide transporter CstA, PEB1 aspartate/glutamate transporter, LutABC lactate dehydrogenase and PutA proline dehydrogenase became more abundant with increasing aerobiosis. We also observed a co-ordinated response of oxidative stress protection enzymes and Fe-S cluster biogenesis proteins above 100% aerobiosis. Our approaches reveal key virulence factors that respond to restricted oxygen availability and specific transporters and catabolic pathways activated with increasing aerobiosis.<br /> (© 2017 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Subjects :
- Campylobacter Infections microbiology
Campylobacter jejuni genetics
Humans
Oxidation-Reduction
Proteome metabolism
Proteomics
Transcriptome genetics
Virulence Factors genetics
Virulence Factors metabolism
Aerobiosis physiology
Campylobacter jejuni metabolism
Campylobacter jejuni pathogenicity
Oxidative Stress physiology
Oxygen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1462-2920
- Volume :
- 19
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28892295
- Full Text :
- https://doi.org/10.1111/1462-2920.13930