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Synthesis, quality control, and bio-evaluation of 99m Tc-cyclophosphamide.
- Source :
-
Chemical biology & drug design [Chem Biol Drug Des] 2018 Feb; Vol. 91 (2), pp. 456-462. Date of Electronic Publication: 2017 Oct 09. - Publication Year :
- 2018
-
Abstract
- Cancer is found to be the leading cause of death worldwide characterized by uncontrolled cell division. Nuclear medicines imaging using radiopharmaceuticals have pronounced potential for the diagnosis and treatment of cancers. Cyclophosphamide (CPH) is an antineoplastic drug which targets selectively cancer cells. In the present work, labeling of CPH with Tc-99m is performed for diagnostic purpose, which gave labeling yield as high as 99% using 20 μg SnCl <subscript>2</subscript> ·2H <subscript>2</subscript> O, 200 μg of ligand at pH 7 for 10 min reaction time at room temperature. The characterization of the prepared complex was performed using ITLC, electrophoresis, and HPLC. In vitro stability was analyzed in the presence of human serum at 37°C which has maximum value of 94 ± 0.5. The biodistribution studies of <superscript>99m</superscript> Tc-CPH were performed in normal and tumor bearing Swiss Webster mice. The high accumulation of <superscript>99m</superscript> Tc-CPH was observed in liver and tumours respectively at 4 hr after injection. Biodistribution results revealed that <superscript>99m</superscript> Tc-CPH may be a potential tumour diagnostic agent simultaneously with chemotherapy.<br /> (© 2017 John Wiley & Sons A/S.)
- Subjects :
- Animals
Antineoplastic Agents administration & dosage
Antineoplastic Agents pharmacokinetics
Cell Line, Tumor
Cyclophosphamide administration & dosage
Cyclophosphamide pharmacokinetics
Drug Stability
Half-Life
Humans
Hydrogen-Ion Concentration
Injections, Intravenous
Mice
Radiopharmaceuticals blood
Technetium chemistry
Tissue Distribution
Transplantation, Heterologous
Whole Body Imaging
Antineoplastic Agents chemistry
Cyclophosphamide chemistry
Radiopharmaceuticals chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1747-0285
- Volume :
- 91
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Chemical biology & drug design
- Publication Type :
- Academic Journal
- Accession number :
- 28886237
- Full Text :
- https://doi.org/10.1111/cbdd.13107