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Global analysis of gene expression profiles in the submandibular salivary gland of klotho knockout mice.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2018 Apr; Vol. 233 (4), pp. 3282-3294. Date of Electronic Publication: 2017 Sep 28. - Publication Year :
- 2018
-
Abstract
- Salivary dysfunction commonly occurs in many older adults and is considered a physiological phenomenon. However, the genetic changes in salivary glands during aging have not been characterized. The present study analyzed the gene expression profile in salivary glands from accelerated aging klotho deficient mice (klotho-/-, 4 weeks old). Microarray analysis showed that 195 genes were differentially expressed (z-score > 2 in two independent arrays) in klotho null mice compared to wild-type mice. Importantly, alpha2-Na <superscript>+</superscript> /K <superscript>+</superscript> -ATPase (Atp1a2), Ca <superscript>2+</superscript> -ATPase (Atp2a1), epidermal growth factor (EGF), and nerve growth factor (NGF), which have been suggested to be regulators of submandibular salivary gland function, were significantly decreased. When a network was constructed from the differentially expressed genes, proliferator-activated receptor-γ (PPAR γ), which regulates energy homeostasis and insulin sensitivity, was located at the core of the network. In addition, the expression of genes proposed to regulate various PPAR γ-related cellular pathways, such as Klk1b26, Egfbp2, Cox8b, Gpx3, Fabp3, EGF, and NGFβ, was altered in the submandibular salivary glands of klotho-/- mice. Our results may provide clues for the identification of novel genes involved in salivary gland dysfunction. Further characterization of these differentially expressed genes will be useful in elucidating the genetic basis of aging-related changes in the submandibular salivary gland.<br /> (© 2017 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc.)
- Subjects :
- Aging metabolism
Animals
Down-Regulation genetics
Gene Ontology
Gene Regulatory Networks
Glucuronidase metabolism
Humans
Klotho Proteins
Mice, Knockout
Oligonucleotide Array Sequence Analysis
PPAR gamma metabolism
Reproducibility of Results
Signal Transduction
Submandibular Gland cytology
Tongue cytology
Transcription Factors metabolism
Up-Regulation genetics
Gene Expression Profiling
Glucuronidase deficiency
Submandibular Gland metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 233
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 28885690
- Full Text :
- https://doi.org/10.1002/jcp.26172