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The role of phospholipase C signaling in bovine herpesvirus 1 infection.
- Source :
-
Veterinary research [Vet Res] 2017 Sep 07; Vol. 48 (1), pp. 45. Date of Electronic Publication: 2017 Sep 07. - Publication Year :
- 2017
-
Abstract
- Bovine herpesvirus 1 (BoHV-1) infection enhanced the generation of inflammatory mediator reactive oxidative species (ROS) and stimulated MAPK signaling that are highly possibly related to virus induced inflammation. In this study, for the first time we show that BoHV-1 infection manipulated phospholipase C (PLC) signaling, as demonstrated by the activation of PLCĪ³-1 at both early stages [at 0.5 h post-infection (hpi)] and late stages (4-12 hpi) during the virus infection of MDBK cells. Viral entry, and de novo protein expression and/or DNA replication were potentially responsible for the activation of PLCĪ³-1 signaling. PLC signaling inhibitors of both U73122 and edelfosine significantly inhibited BoHV-1 replication in both bovine kidney cells (MDBK) and rabbit skin cells (RS-1) in a dose-dependent manner by affecting the virus entry stage(s). In addition, the activation of Erk1/2 and p38MAPK signaling, and the enhanced generation of ROS by BoHV-1 infection were obviously ameliorated by chemical inhibition of PLC signaling, implying the requirement of PLC signaling in ROS production and these MAPK pathway activation. These results suggest that the activation of PLC signaling is a potential pathogenic mechanism for BoHV-1 infection.
- Subjects :
- Animals
Cattle
Flow Cytometry veterinary
Herpesviridae Infections virology
MAP Kinase Signaling System physiology
Reactive Oxygen Species metabolism
Virus Replication
Cattle Diseases virology
Herpesviridae Infections veterinary
Herpesvirus 1, Bovine physiology
Signal Transduction physiology
Type C Phospholipases physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1297-9716
- Volume :
- 48
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary research
- Publication Type :
- Academic Journal
- Accession number :
- 28882164
- Full Text :
- https://doi.org/10.1186/s13567-017-0450-5