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Identification of senescent cell surface targetable protein DPP4.
- Source :
-
Genes & development [Genes Dev] 2017 Aug 01; Vol. 31 (15), pp. 1529-1534. Date of Electronic Publication: 2017 Sep 06. - Publication Year :
- 2017
-
Abstract
- Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts to eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, the differential presence of DPP4 allowed flow cytometry-mediated isolation of senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cytotoxicity (ADCC) assays revealed that the cell surface DPP4 preferentially sensitized senescent, but not dividing, fibroblasts to cytotoxicity by natural killer cells. In sum, the selective expression of DPP4 on the surface of senescent cells enables their preferential elimination.<br /> (© 2017 Kim et al.; Published by Cold Spring Harbor Laboratory Press.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Antibody-Dependent Cell Cytotoxicity
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p16 genetics
Diploidy
Fibroblasts metabolism
Flow Cytometry
Humans
Killer Cells, Natural metabolism
Lymphocyte Subsets enzymology
Mass Spectrometry
RNA, Messenger metabolism
RNA, Ribosomal metabolism
Cellular Senescence physiology
Dipeptidyl Peptidase 4 metabolism
Membrane Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1549-5477
- Volume :
- 31
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Genes & development
- Publication Type :
- Academic Journal
- Accession number :
- 28877934
- Full Text :
- https://doi.org/10.1101/gad.302570.117