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Ecdysteroids during embryonation of eggs of Ascaris suum.
- Source :
-
Comparative biochemistry and physiology. A, Comparative physiology [Comp Biochem Physiol A Comp Physiol] 1987; Vol. 87 (3), pp. 803-5. - Publication Year :
- 1987
-
Abstract
- 1. The optimal temperature for in vitro development of fertilized eggs of Ascaris suum was 24 degrees C. 2. Samples (2 X 10(7) eggs) were obtained from in vitro embryonating cultures every 3 days for 4 weeks; lipids were extracted, partially purified, fractionated with HPLC and analyzed for ecdysteroids by radioimmunoassay. 3. Free ecdysone and 20-hydroxyecdysone (20-HE) were at low levels (less than 20 pg) in freshly excised eggs and rose to maximal values on day 6 of embryonation. 4. Conjugated ecdysone and conjugated 20-HE rose to maximal values on day 9. 5. Both free and conjugated ecdysteroids were undetectable from days 15 to 27 of cultivation. 6. These profiles indicate that ecdysteroids might have a selective role in nematode embryonation and/or tanning of the egg shell.
Details
- Language :
- English
- ISSN :
- 0300-9629
- Volume :
- 87
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. A, Comparative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 2887364
- Full Text :
- https://doi.org/10.1016/0300-9629(87)90404-x