[Expression of BRCA2 in tumor tissues in patients with laryngeal squamous cell carcinoma and its clinical significance].

Objective To determine the role of breast cancer susceptibility gene 2 (BRCA2) in laryngeal squamous cell carcinoma and investigate its clinical implication. Methods The expression of BRCA2 was examined by immunohistochemistry in 62 tissues of pathologically identified laryngeal squamous cell carcinoma and 23 tissues of vocal fold polyp. The relationships between BRCA2 expression and clinicopathological characteristics were further analyzed. The expression of BRCA2 mRNA and protein were detected by real-time quantitative PCR (qRT-PCR) and Western blot analysis in pathologically identified laryngeal squamous cell carcinoma tissues and corresponding normal tissues. Results The immunohistochemistry showed that the expression rate of BRCA2 in laryngeal carcinoma tissues and vocal fold polyp tissues were 29.03%(18/62) and 69.56%(16/23), respectively. The expression of BRCA2 was not significantly related with patients' gender, age, smoking, differentiation degree, cervical lymph node metastasis, and clinical stage. There was a negative correlation between BRCA2 expression and T stage. The qRT-PCR and Western blotting showed that BRCA2 mRNA and protein expression in the laryngeal squamous carcinoma tissues was lower than that in the corresponding normal tissues. Conclusion The expression of BRCA2 is low in laryngeal squamous cell carcinoma, and it is negatively correlated with T stage.