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The HIV aspartyl protease inhibitor ritonavir impairs planktonic growth, biofilm formation and proteolytic activity in Trichosporon spp.
- Source :
-
Biofouling [Biofouling] 2017 Sep; Vol. 33 (8), pp. 640-650. - Publication Year :
- 2017
-
Abstract
- This study evaluated the effect of the protease inhibitor ritonavir (RIT) on Trichosporon asahii and Trichosporon inkin. Susceptibility to RIT was assessed by the broth microdilution assay and the effect of RIT on protease activity was evaluated using azoalbumin as substrate. RIT was tested for its anti-biofilm properties and RIT-treated biofilms were assessed regarding protease activity, ultrastructure and matrix composition. In addition, antifungal susceptibility, surface hydrophobicity and biofilm formation were evaluated after pre-incubation of planktonic cells with RIT for 15 days. RIT (200 μg ml <superscript>-1</superscript> ) inhibited Trichosporon growth. RIT (100 μg ml <superscript>-1</superscript> ) also reduced protease activity of planktonic and biofilm cells, decreased cell adhesion and biofilm formation, and altered the structure of the biofilm and the protein composition of the biofilm matrix. Pre-incubation with RIT (100 μg ml <superscript>-1</superscript> ) increased the susceptibility to amphotericin B, and reduced surface hydrophobicity and cell adhesion. These results highlight the importance of proteases as promising therapeutic targets and reinforce the antifungal potential of protease inhibitors.
- Subjects :
- Biofilms growth & development
Drug Interactions
Microbial Sensitivity Tests
Peptide Hydrolases metabolism
Plankton growth & development
Plankton metabolism
Trichosporon growth & development
Trichosporon metabolism
Antifungal Agents pharmacology
Biofilms drug effects
HIV Protease Inhibitors pharmacology
Plankton drug effects
Ritonavir pharmacology
Trichosporon drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2454
- Volume :
- 33
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Biofouling
- Publication Type :
- Academic Journal
- Accession number :
- 28871863
- Full Text :
- https://doi.org/10.1080/08927014.2017.1350947