Back to Search
Start Over
Analysis of stress factors associated with KHV reactivation and pathological effects from KHV reactivation.
- Source :
-
Virus research [Virus Res] 2017 Aug 15; Vol. 240, pp. 200-206. Date of Electronic Publication: 2017 Aug 30. - Publication Year :
- 2017
-
Abstract
- Koi herpesvirus (KHV) is a highly pathogenic virus of common carp and koi. KHV becomes latent in recovered koi or exposed koi without symptoms, and the latent infection can reactivate under stress conditions. KHV reactivation from latency often occurs when water temperature rapidly rises above 17°C. Dissolved O <subscript>2</subscript> is lower at ≥17°C than at non-stress temperatures ≤15°C. To determine whether reduced dissolved O <subscript>2</subscript> level has a role in KHV reactivation during temperature stress, KHV reactivation was investigated in KHV latently infected koi (KHV <superscript>+</superscript> koi) under stress temperatures by maintaining dissolved O <subscript>2</subscript> consistent with the O <subscript>2</subscript> level at 15°C. There was no significant difference in the amount of reactivated virus between KHV <superscript>+</superscript> koi maintained with and without O <subscript>2</subscript> supplementation during temperature stress. Both handling and sampling were found to be stressful to koi and can contribute to KHV reactivation from latency. There was an increase in KHV genome within white blood cells (WBC) during KHV reactivation, which is about 3-4 fold higher than the amount of KHV genome detectable in WBC during the latency stage. At day 15 post-temperature stress (PTS), inflammation and necrosis were observed in multiple tissues, especially in the gills, eye, intestine, skin and kidney. KHV DNA was also detectable in multiple tissues on days 6, 9 and 15 PTS. Following day 3 PTS, the plasma cortisol levels were higher than that observed in koi before temperature stress, suggesting that KHV reactivation is associated with physiological stress in KHV <superscript>+</superscript> koi.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Carps physiology
Carps virology
Fish Diseases pathology
Fish Diseases physiopathology
Herpesviridae genetics
Herpesviridae isolation & purification
Herpesviridae Infections pathology
Herpesviridae Infections physiopathology
Herpesviridae Infections virology
Stress, Physiological
Temperature
Virus Latency
Fish Diseases virology
Herpesviridae physiology
Herpesviridae Infections veterinary
Virus Activation
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7492
- Volume :
- 240
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 28860099
- Full Text :
- https://doi.org/10.1016/j.virusres.2017.08.010