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The INO80 remodeller in transcription, replication and repair.
- Source :
-
Philosophical transactions of the Royal Society of London. Series B, Biological sciences [Philos Trans R Soc Lond B Biol Sci] 2017 Oct 05; Vol. 372 (1731). - Publication Year :
- 2017
-
Abstract
- The accessibility of eukaryotic genomes to the action of enzymes involved in transcription, replication and repair is maintained despite the organization of DNA into nucleosomes. This access is often regulated by the action of ATP-dependent nucleosome remodellers. The INO80 class of nucleosome remodellers has unique structural features and it is implicated in a diverse array of functions, including transcriptional regulation, DNA replication and DNA repair. Underlying these diverse functions is the catalytic activity of the main ATPase subunit, which in the context of a multisubunit complex can shift nucleosomes and carry out histone dimer exchange. In vitro studies showed that INO80 promotes replication fork progression on a chromatin template, while in vivo it was shown to facilitate replication fork restart after stalling and to help evict RNA polymerase II at transcribed genes following the collision of a replication fork with transcription. More recent work in yeast implicates INO80 in the general eviction and degradation of nucleosomes following high doses of oxidative DNA damage. Beyond these replication and repair functions, INO80 was shown to repress inappropriate transcription at promoters in the opposite direction to the coding sequence. Here we discuss the ways in which INO80's diverse functions help maintain genome integrity.This article is part of the themed issue 'Chromatin modifiers and remodellers in DNA repair and signalling'.<br /> (© 2017 The Author(s).)
- Subjects :
- Chromatin genetics
Chromatin metabolism
Chromatin Assembly and Disassembly
Gene Expression Regulation
Saccharomyces cerevisiae Proteins metabolism
Transcription Factors metabolism
DNA Repair
DNA Replication
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Transcription Factors genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2970
- Volume :
- 372
- Issue :
- 1731
- Database :
- MEDLINE
- Journal :
- Philosophical transactions of the Royal Society of London. Series B, Biological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28847827
- Full Text :
- https://doi.org/10.1098/rstb.2016.0290