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Metabonomics screening of serum identifies pyroglutamate as a diagnostic biomarker for nonalcoholic steatohepatitis.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2017 Oct; Vol. 473, pp. 89-95. Date of Electronic Publication: 2017 Aug 23. - Publication Year :
- 2017
-
Abstract
- Objective: A key step in managing non-alcoholic fatty liver disease (NAFLD) is to differentiate nonalcoholic steatohepatitis (NASH) from simple steatosis (SS).<br />Method: Serum samples were collected from three groups: NASH patients (N=21), SS patients (N=38) and healthy controls (N=31). High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to analyse the metabolic profile of the serum samples. The acquired data were processed by multivariate principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) to identify novel metabolites. The potential biomarkers were quantitatively determined and their diagnostic power was further validated.<br />Results: A total of 56 metabolites were capable of distinguishing NASH from SS samples based on the OPLS-DA model. Pyroglutamate was found to be the most promising factor in distinguishing the NASH from SS groups. With an optimal cut-off value of 4.82mmol/L, the sensitivity and specificity of the diagnosis of NASH were 72% and 85%, respectively. The area under the receiver operating characteristic (AUROC) of the pyroglutamate levels of NASH versus SS patients was more than those of tumor necrosis factor-α, adiponectin and interleukin-8.<br />Conclusion: These data suggest that pyroglutamate may be a new and useful biomarker for the diagnosis of NASH.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3492
- Volume :
- 473
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28842175
- Full Text :
- https://doi.org/10.1016/j.cca.2017.08.022