Back to Search
Start Over
Cloning and characterization of an insect apolipoprotein (apolipophorin-II/I) involved in the host immune response of Antheraea pernyi.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2017 Dec; Vol. 77, pp. 221-228. Date of Electronic Publication: 2017 Aug 19. - Publication Year :
- 2017
-
Abstract
- Apolipoproteins are protein components of lipoprotein particles, and are increasingly recognized to be functioning in the innate immune systems of both insects and mammals. Mammalian apolipoprotein B (apoB) is associated with a diverse range of innate immune defenses including suppression of bacterial pathogenesis, virus toxicity neutralization, and inhibition of cytokine releases from immune cells. However, little is known about apoB homologous insect apolipophorin-II/I (apoLp-II/I) in controlling of specific pathogen-host encounters such as microbial infections. In the present study, we describe cDNA cloning and characterization of an apoLp-II/I from Chinese oak silk worm, Antheraea pernyi. The apoLp-II/I cDNA is 10237bp in length, which possesses an open reading frame encoding 3305 amino acids. A consensus cleavage site RGRR presenting from Arg <superscript>710</superscript> to Arg <superscript>713</superscript> implies posttranslational cleavage of this protein. Ap-apoLp-II/I shares high sequence identities with apoLp-II/I in lepidoptera and other insects. In addition, considerable similarities also exist between Ap-apoLp-II/I and human apoB, which basically positioned in first 1000 residues of the amino termini. Tissue distribution and time-course expression results demonstrate that Ap-apoLp-II/I transcripts were detected predominantly in the fat body, less in epidermis and rarely in midgut, while the synthetic apoLp-II/I protein was abundant in hemocytes and plasma instead of the fatbody. Expression of Ap-apoLp-II/I was stimulated in response to bacterial challenge. In addition, our preliminary studies established a novel role for Ap-apoLp-II/I in regulating prophenoloxidase activation system. Taken together, apoLp-II/I may play an essential role in innate responses of Antheraea pernyi.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Apolipoproteins metabolism
Cloning, Molecular
Host-Pathogen Interactions
Immunity, Innate
Insect Proteins metabolism
Phylogeny
Protein Processing, Post-Translational
Sequence Alignment
Serine Endopeptidases metabolism
Transcriptome
Apolipoproteins genetics
Bacterial Infections immunology
Fat Body physiology
Insect Proteins genetics
Moths immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 77
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28830681
- Full Text :
- https://doi.org/10.1016/j.dci.2017.08.010