Phytoactinopolyspora halotolerans sp. nov., a halotolerant actinobacterium isolated from a saline soil in Xinjiang, northwest of China.

A novel aerobic, Gram-positive, non-motile halotolerant actinobacterium, designated strain YIM 96448 ^T , was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96448 ^T is closely related to Phytoactinopolyspora endophytica EGI 60009 ^T (98.0%) and Phytoactinopolyspora alkaliphila EGI 80629 ^T (97.0%). Optimum growth of the strain was observed at 28-37 °C, pH 7-9 and in the presence of 5-8% NaCl (w/v). The respiratory quinone of strain YIM 96448 ^T was determined to be MK-9(H ₄ ). The major fatty acids (>10%) were identified as anteiso-C _15:0 , iso-C _16:0 , anteiso-C _17:0 and summed feature 4 (iso-C _17:1 I and/or anteiso-C _17:1 B). The polar lipid profile was found to consist of diphosphatidylglycerol, an unidentified aminophospholipid, phosphatidylglycerol, two phosphatidylinositol mannosides, three unidentified phosphoglycolipids and four unidentified phospholipids. The DNA G+C content was determined to be 68.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain YIM 96448 ^T should be classified as a novel species in the genus Phytoactinopolyspora, for which the name Phytoactinopolyspora halotolerans sp. nov. is proposed. The type strain is YIM 96448 ^T (=CCTCC AB 2017055 ^T  = KCTC 39924 ^T ).