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BCG Increased Membrane Expression of TRIM59 Through the TLR2/ TLR4/IRF5 Pathway in RAW264.7 Macrophages.
- Source :
-
Protein and peptide letters [Protein Pept Lett] 2017; Vol. 24 (8), pp. 765-770. - Publication Year :
- 2017
-
Abstract
- Objective: In our previous study, we showed that Bacillus Calmette-Guerin (BCG)- activated macrophages have the ability to directly kill tumor cells. One of the main properties of these macrophages is the high expression of tripartite motif family protein 59 (TRIM59). This study was conducted to investigate the mechanism of BCG-induced TRIM59 expression on macrophages and to identify the subcellular localization of TRIM59.<br />Methods: TRIM59 expression and TNF-α secretion were compared in RAW264.7 macrophage cells that were stimulated using BCG with or without Toll-like receptor 2/4 (TLR2/4)-neutralizing antibodies. Next, small interfering RNA (siRNA) was used to down-regulated interferon regulatory factor 5 (IRF5) gene expression in RAW264.7 cells. Transfected cells were stimulated with BCG, after which TRIM59 expression and TNF-α secretion were evaluated in cells pre-treated with siRNA or scramble control. After treatments, supernatants were co-cultured with MCA207, and cell viabilities were determined. Moreover, BCG-stimulated RAW264.7 cells were stained for TRIM59 and F4/80 expression.<br />Results: In this study, we showed that TRIM59 was expressed on the membrane of RAW264.7 cells. After blocking TLR2/4, treatment with BCG failed to induce the expression of TRIM59, IRF5, and TNF-α on RAW264.7 cells. In addition, down-regulation of IRF5 inhibited TRIM59 and TNF-α expression.<br />Conclusion: Our study showed that TRIM59 is a membrane protein, and that BCG treatment upregulated TRIM59 expression on macrophages via TLR2/4 and IRF5 pathways.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Subjects :
- Animals
Antibodies, Neutralizing pharmacology
Carrier Proteins metabolism
Cell Line
Cell Line, Tumor
Cell Survival drug effects
Culture Media, Conditioned isolation & purification
Culture Media, Conditioned pharmacology
Gene Expression Regulation
Humans
Interferon Regulatory Factors antagonists & inhibitors
Interferon Regulatory Factors metabolism
Intracellular Signaling Peptides and Proteins
Macrophages cytology
Macrophages metabolism
Macrophages microbiology
Mice
Mycobacterium bovis physiology
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Signal Transduction
Toll-Like Receptor 2 antagonists & inhibitors
Toll-Like Receptor 2 metabolism
Toll-Like Receptor 4 antagonists & inhibitors
Toll-Like Receptor 4 metabolism
Tripartite Motif Proteins
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Carrier Proteins genetics
Interferon Regulatory Factors genetics
Mycobacterium bovis chemistry
Toll-Like Receptor 2 genetics
Toll-Like Receptor 4 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1875-5305
- Volume :
- 24
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Protein and peptide letters
- Publication Type :
- Academic Journal
- Accession number :
- 28820065
- Full Text :
- https://doi.org/10.2174/0929866524666170818155524