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Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter).
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1634, pp. 153-162. - Publication Year :
- 2017
-
Abstract
- Circulating tumor cells (CTCs) are currently widely studied for their potential application as part of a liquid biopsy. These cells are shed from the primary tumor into the circulation, and are postulated to provide insight into the molecular makeup of the actual tumor in a minimally invasive manner. However, they are extremely rare in blood, with typical concentrations of 1-100 in a milliliter of blood; hence, a need exists for a rapid and high-purity method for isolating CTCs from whole blood. Here, we describe the application of a microfabricated magnetic sifter toward isolation of CTCs from whole blood at volumetric flow rates of 10 mL/h, along with the use of a PDMS-based nanowell system for single-cell gene expression profiling. This method allows rapid isolation of CTCs and subsequent integration with downstream genetic profiling methods for clinical applications such as targeted therapy, therapy monitoring, or further biological studies.
- Subjects :
- Cell Line, Tumor
Cell Separation instrumentation
Cell Size
Dimethylpolysiloxanes chemistry
Equipment Design
Filtration instrumentation
Gene Expression Profiling
Humans
Lung Neoplasms blood
Lung Neoplasms pathology
Magnetite Nanoparticles chemistry
Magnets
Nanopores
Neoplastic Cells, Circulating metabolism
Nylons chemistry
Rheology
Single-Cell Analysis instrumentation
Cell Separation methods
Filtration methods
Gene Expression Regulation, Neoplastic
Lung Neoplasms diagnosis
Neoplastic Cells, Circulating pathology
Single-Cell Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1634
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 28819848
- Full Text :
- https://doi.org/10.1007/978-1-4939-7144-2_12