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The possible molecular mechanisms of bisphenol A action on porcine early embryonic development.
- Source :
-
Scientific reports [Sci Rep] 2017 Aug 17; Vol. 7 (1), pp. 8632. Date of Electronic Publication: 2017 Aug 17. - Publication Year :
- 2017
-
Abstract
- Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated the role and mechanism of BPA in porcine embryonic development. First, the parthenotes were treated with different concentrations of BPA. We found that blastocyst formation was impaired and the parthenotes were arrested at the 4-cell stage after treatment with 100 μm BPA. Second, ROS increased following the addition of BPA, which further caused mitochondrial damage, and cytochrome c was released from the mitochondria to induce apoptosis. The adaptive response was demonstrated through LC3 immunofluorescence staining and by assessing autophagy-related gene expression. In addition, BPA caused DNA damage through the p53-p21 signaling pathway. Thus, our results indicate that BPA displays an adverse effect on porcine early embryonic development through mitochondrial and DNA damage.
- Subjects :
- Animals
Apoptosis drug effects
Apoptosis genetics
Autophagy drug effects
Autophagy genetics
Blastocyst cytology
Blastocyst metabolism
Cytochromes c metabolism
Dose-Response Relationship, Drug
Embryonic Development genetics
Estrogens, Non-Steroidal pharmacology
Gene Expression drug effects
Mitochondria metabolism
Reactive Oxygen Species metabolism
Signal Transduction drug effects
Signal Transduction genetics
Swine
Benzhydryl Compounds pharmacology
Blastocyst drug effects
Embryonic Development drug effects
Mitochondria drug effects
Phenols pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28819136
- Full Text :
- https://doi.org/10.1038/s41598-017-09282-2