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The isotype and IgG subclass distribution of anti-carbamylated protein antibodies in rheumatoid arthritis patients.
- Source :
-
Arthritis research & therapy [Arthritis Res Ther] 2017 Aug 15; Vol. 19 (1), pp. 190. Date of Electronic Publication: 2017 Aug 15. - Publication Year :
- 2017
-
Abstract
- Background: Anti-carbamylated protein (anti-CarP) antibodies have recently been reported to occur in around 45% of rheumatoid arthritis (RA) patients and to have prognostic and diagnostic properties. At present, the breadth and molecular make-up of the anti-CarP antibody response is ill defined. To understand the anti-CarP antibody immune response and potential immune effector mechanisms it can recruit, we determined the anti-CarP antibody isotype and IgG-subclass usage in RA patients.<br />Methods: Anti-CarP antibody IgM, IgA, and IgG or IgG subclasses were detected by enzyme-linked immunosorbent assay (ELISA) in sera from 373 unselected RA patients and 196 healthy controls. An additional 114 anti-citrullinated protein antibody (ACPA) and anti-CarP IgG double-positive patients were selected to study the concomitant presence of both antibody systems.<br />Results: Anti-CarP IgG was present in around 45% of the patients and comprised all anti-CarP IgG subclasses. The presence of anti-CarP IgG1 particularly associates with radiological damage. Anti-CarP IgM was detected in 16% of RA patients, even in anti-CarP IgG-positive individuals, and is indicative of an actively ongoing immune response. Around 45% of the patients were positive for IgA which included ACPA-positive cases but also 24% of the ACPA-negative cases. In ACPA and anti-CarP double-positive patients, the distribution and number of isotypes and IgG subclasses was similar for both autoantibodies at the group level, but substantial variation was observed within individual patient samples.<br />Conclusions: In RA, the anti-CarP antibody response uses a broad spectrum of isotypes and seems to be an actively ongoing immune reaction. Furthermore, the anti-CarP and ACPA autoantibody responses seems to be differentially regulated.
- Subjects :
- Adult
Aged
Anti-Citrullinated Protein Antibodies blood
Anti-Citrullinated Protein Antibodies immunology
Anti-Citrullinated Protein Antibodies metabolism
Arthritis, Rheumatoid blood
Arthritis, Rheumatoid metabolism
Autoantibodies blood
Autoantibodies metabolism
Carbamates metabolism
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G blood
Immunoglobulin G metabolism
Immunoglobulin Isotypes blood
Immunoglobulin Isotypes metabolism
Male
Middle Aged
Proteins metabolism
Arthritis, Rheumatoid immunology
Autoantibodies immunology
Carbamates immunology
Immunoglobulin G immunology
Immunoglobulin Isotypes immunology
Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1478-6362
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Arthritis research & therapy
- Publication Type :
- Academic Journal
- Accession number :
- 28810902
- Full Text :
- https://doi.org/10.1186/s13075-017-1392-z