Back to Search
Start Over
Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1651, pp. 213-227. - Publication Year :
- 2017
-
Abstract
- Activity of endogenous promoters can be altered by including additional responsive elements (REs). These elements can be responsive to features of the tumor environment or alternatively to signaling pathways specifically activated in cancer cells. These REs incorporated into tumor-specific promoters can improve cancer targeting, the replicative capacity, and lytic activity of conditionally replicative adenovirus. Here we outline an approach to incorporate hypoxia and inflammation REs into a specific fragment of the SPARC promoter and the steps to clone a nucleosome positioning sequence (NPS ) identified in the osteocalcin promoter that contains a Wnt RE upstream of a heterologous synthetic promoter.
- Subjects :
- Animals
Cell Line, Tumor
Cloning, Molecular methods
Gene Expression Regulation
HEK293 Cells
Humans
Hypoxia genetics
Inflammation genetics
Neoplasms metabolism
Osteocalcin genetics
Plasmids genetics
Rats
Wnt Signaling Pathway
Adenoviridae genetics
Neoplasms genetics
Oncolytic Viruses genetics
Promoter Regions, Genetic
Tumor Microenvironment
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1651
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 28801910
- Full Text :
- https://doi.org/10.1007/978-1-4939-7223-4_16