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Endoplasmic Reticulum Stress Inducer Tunicamycin Alters Hepatic Energy Homeostasis in Mice.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Aug 04; Vol. 18 (8). Date of Electronic Publication: 2017 Aug 04. - Publication Year :
- 2017
-
Abstract
- Disorders of hepatic energy metabolism, which can be regulated by endoplasmic reticulum (ER) stress, lead to metabolic diseases such as hepatic steatosis and hypoglycemia. Tunicamycin, a pharmacological ER stress inducer, is used to develop an anti-cancer drug. However, the effects of tunicamycin on hepatic energy metabolism have not been well elucidated. Mice were intraperitoneally injected with tunicamycin or vehicle. Twenty-four hours later, hepatic triglyceride and glycogen content and serum lipids profiles were analyzed, as well as the expression of lipogenic and gluconeogenic genes. Tunicamycin significantly induced hepatic a yellowish color and ER stress, as well as increasing serum levels of aspartate transaminase and alanine transaminase. Besides, tunicamycin remarkably increased hepatic triglyceride content and suppressed the expression of apolipoprotein B100. In addition, tunicamycin-treated mice had lower serum levels of triglyceride, apolipoprotein B, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol. Gene expression of peroxisome proliferator-activated receptor α was decreased by tunicamycin, but the protein level was increased. Furthermore, blood glucose level and hepatic glycogen content were decreased in tunicamycin-treated mice. Protein kinase B signaling was attenuated in the tunicamycin-treated liver, but the expression and activities of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase were unchanged. Tunicamycin alters hepatic energy homeostasis by increasing triglyceride accumulation and decreasing glycogen content.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Blood Glucose metabolism
Endoplasmic Reticulum Stress genetics
Energy Metabolism genetics
Gene Expression Regulation drug effects
Glycogen metabolism
Lipogenesis drug effects
Lipogenesis genetics
Lipoproteins metabolism
Liver drug effects
Male
Mice, Inbred C57BL
Triglycerides metabolism
Tunicamycin administration & dosage
Endoplasmic Reticulum Stress drug effects
Energy Metabolism drug effects
Homeostasis drug effects
Liver metabolism
Tunicamycin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28777337
- Full Text :
- https://doi.org/10.3390/ijms18081710